Benedict's Test - Qualitative Test in Carbohydrates

Overview

This programme demonstrates in a video lesson, the procedure in the Qualitative test for Carbohydrates. In this Biochemistry Laboratory Test, the Benedict's test procedure is demonstrated. Benedict's test distinguishes reducing sugars from non reducing sugars. The procedure covers the description of the laboratory instruments and materials that are used in such tests.

 

At the end of the procedure, an observation of the test result is made and interpreted and a well balanced reaction equation is obtained.

Author(s): George KoffuorView Details of Author
Year: 2012
Keywords: Benedict's test, reducing sugars, non reducing sugars,
Licensing Condition:

cc-by-nc© 2012, KNUST

Unless otherwise noted, the content of this programme is licensed under the terms of a Creative Commons Attribution-NonCommercial 3.0 Unported License.

Disclaimer

 

Any information and/or video demonstration in this material is intended to inform and to educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. You should speak to your lecturer, healthcare professional or lab technician and make an appointment, if you have questions or concerns about this information.

 

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Introduction

 

Benedict's test distinguishes reducing sugars from non reducing sugars. Heating a mixture of Benedict's qualitative reagent and a reducing sugar such as gluscose in a basic medium (pH 10.5) leads to the reduction of cupric ions in Benedict's qualitative reagent to cuprous ions, with the formation of copper (I) oxide seen as a yellow to red precipitate in the mixture.

 

Objectives

 

Distinguish between reducing and non reducing sugars using the Benedict's test.

Outline the test process.

Write a well balanced reaction equation for the test.

 

Lesson Content

Benedict's Test (video)
Benedict's Test

References

     

    This programme was produced with support from the following:

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Credits

 

Author

gkoff

Dr. George Koffuor
Department of Pharmacology
College of Health Sciences - KNUST
Kumasi, Ghana

Student Volunteer

christola

Christola Lord Acheampong
Department of Communication Design
CASS - KNUST
Kumasi, Ghana

 

OER Technical Support

 

 

 

Benjamin Prempeh
OER Media Specialist
KNUST, Kumasi - Ghana
adobrog@yahoo.com

John Kofi Dogbey
Media Specialist
KNUST,Kumasi - Ghana
johndogbey@gmail.com


Voice Over

ella kasanga

Ella Kasanga
Kumasi, Ghana